target-specific sirna Search Results


target-specific sirnas of rat nfe212 #83619-1  (Bioneer Corporation)
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double-stranded hmgn5 -targeted sirnas pscsi616  (Genechem)
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Genechem double-stranded hmgn5 -targeted sirnas pscsi616
Double Stranded Hmgn5 Targeted Sirnas Pscsi616, supplied by Genechem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cdna encoding full-length human sphk1  (Shanghai GenePharma)
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Cdna Encoding Full Length Human Sphk1, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sirna specifically targeting rora  (Ribobio co)
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Sirna Specifically Targeting Rora, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sirnas targeting lnc rna dancr and non-specific control (nc)  (Ribobio co)
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Sirnas Targeting Lnc Rna Dancr And Non Specific Control (Nc), supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fulllength brg1 sequences  (Shanghai GenePharma)
90
Shanghai GenePharma fulllength brg1 sequences
Fulllength Brg1 Sequences, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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small interfering rna against ccat2  (Shanghai GenePharma)
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Small Interfering Rna Against Ccat2, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sh-ddx11-as1  (Shanghai GenePharma)
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Primer sequence
Sh Ddx11 As1, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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target-specific sirna oligo duplexes sipbgd  (MyBiosource Biotechnology)
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Primer sequence
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three specific sirnas which were designed targeting the back-splice junction site of circash2l  (Ribobio co)
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Ribobio co three specific sirnas which were designed targeting the back-splice junction site of circash2l
Primer sequence
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pp-1 β sirna  (Ribobio co)
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Ribobio co pp-1 β sirna
Primer sequence
Pp 1 β Sirna, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gpx3-target specific small interfering rna (sirna)  (Shanghai GenePharma)
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Shanghai GenePharma gpx3-target specific small interfering rna (sirna)
Primer sequence
Gpx3 Target Specific Small Interfering Rna (Sirna), supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Primer sequence

Journal: Cancer Cell International

Article Title: Silenced lncRNA DDX11-AS1 or up-regulated microRNA-34a-3p inhibits malignant phenotypes of hepatocellular carcinoma cells via suppression of TRAF5

doi: 10.1186/s12935-021-01847-6

Figure Lengend Snippet: Primer sequence

Article Snippet: The lentivirus containing sh-DDX11-AS1 and sh-NC, miR-34a-3p mimic and its NC were purchased from GenePharma Co. Ltd. (Shanghai, China). pcDDX11-AS1 was constructed by RiboBio Co., Ltd. (Guangdong, China).

Techniques:

Elevated DDX11-AS1, TRAF5 and reduced miR-34a-3p are present in HCC tissues and cells. a DDX11-AS1, miR-34a-3p and TRAF5 expression in HCC tissues and adjacent tissues detected via RT-qPCR; b TRAF5 protein bands in HCC tissues and adjacent tissues; c TRAF5 protein expression in HCC tissues and adjacent tissues detected via western blot analysis; d The correlation between DDX11-AS1 and miR-34a-3p expression in HCC patients analyzed via Pearson test; e The correlation between DDX11-AS1 and TRAF5 expression in HCC patients analyzed via Pearson test; f The correlation between miR-34a-3p and TRAF5 expression in HCC patients analyzed via Pearson test; g DDX11-AS1, miR-34a-3p and TRAF5 expression in normal human hepatocytes LO2 and HCC cell lines SMMC-7721 and SK-hep1 detected via RT-qPCR; h TRAF5 protein bands in normal human hepatocytes LO2 and HCC cell lines SMMC-7721 and SK-hep1; i TRAF5 protein expression in normal human hepatocytes LO2 and HCC cell lines SMMC-7721 and SK-hep1 detected via western blot analysis; The measurement data were expressed as mean ± standard deviation. The t test was used for two-group comparison. One-way ANOVA was used for comparison among multiple groups, and Tukey’s post hoc test was used for pairwise comparison. Pearson test was used for correlation analysis. * vs LO2 cells, P < 0.05

Journal: Cancer Cell International

Article Title: Silenced lncRNA DDX11-AS1 or up-regulated microRNA-34a-3p inhibits malignant phenotypes of hepatocellular carcinoma cells via suppression of TRAF5

doi: 10.1186/s12935-021-01847-6

Figure Lengend Snippet: Elevated DDX11-AS1, TRAF5 and reduced miR-34a-3p are present in HCC tissues and cells. a DDX11-AS1, miR-34a-3p and TRAF5 expression in HCC tissues and adjacent tissues detected via RT-qPCR; b TRAF5 protein bands in HCC tissues and adjacent tissues; c TRAF5 protein expression in HCC tissues and adjacent tissues detected via western blot analysis; d The correlation between DDX11-AS1 and miR-34a-3p expression in HCC patients analyzed via Pearson test; e The correlation between DDX11-AS1 and TRAF5 expression in HCC patients analyzed via Pearson test; f The correlation between miR-34a-3p and TRAF5 expression in HCC patients analyzed via Pearson test; g DDX11-AS1, miR-34a-3p and TRAF5 expression in normal human hepatocytes LO2 and HCC cell lines SMMC-7721 and SK-hep1 detected via RT-qPCR; h TRAF5 protein bands in normal human hepatocytes LO2 and HCC cell lines SMMC-7721 and SK-hep1; i TRAF5 protein expression in normal human hepatocytes LO2 and HCC cell lines SMMC-7721 and SK-hep1 detected via western blot analysis; The measurement data were expressed as mean ± standard deviation. The t test was used for two-group comparison. One-way ANOVA was used for comparison among multiple groups, and Tukey’s post hoc test was used for pairwise comparison. Pearson test was used for correlation analysis. * vs LO2 cells, P < 0.05

Article Snippet: The lentivirus containing sh-DDX11-AS1 and sh-NC, miR-34a-3p mimic and its NC were purchased from GenePharma Co. Ltd. (Shanghai, China). pcDDX11-AS1 was constructed by RiboBio Co., Ltd. (Guangdong, China).

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Standard Deviation

Reduced DDX11-AS1 inhibits the proliferation, promotes apoptosis of HCC cells. a The cell growth curve detected by CCK-8 assay; b The cell proliferation rate detected via colony formation assay; c The apoptosis rate detected via Annexin V-FITC/PI double staining; The measurement data were expressed as mean ± standard deviation. The t test was used for two-group comparison. ^ vs the sh-NC group, P < 0.05

Journal: Cancer Cell International

Article Title: Silenced lncRNA DDX11-AS1 or up-regulated microRNA-34a-3p inhibits malignant phenotypes of hepatocellular carcinoma cells via suppression of TRAF5

doi: 10.1186/s12935-021-01847-6

Figure Lengend Snippet: Reduced DDX11-AS1 inhibits the proliferation, promotes apoptosis of HCC cells. a The cell growth curve detected by CCK-8 assay; b The cell proliferation rate detected via colony formation assay; c The apoptosis rate detected via Annexin V-FITC/PI double staining; The measurement data were expressed as mean ± standard deviation. The t test was used for two-group comparison. ^ vs the sh-NC group, P < 0.05

Article Snippet: The lentivirus containing sh-DDX11-AS1 and sh-NC, miR-34a-3p mimic and its NC were purchased from GenePharma Co. Ltd. (Shanghai, China). pcDDX11-AS1 was constructed by RiboBio Co., Ltd. (Guangdong, China).

Techniques: CCK-8 Assay, Colony Assay, Double Staining, Standard Deviation

Reduced DDX11-AS1 inhibits the migration, invasion of HCC cells, promotes and represses the tumor growth in nude mice. a The cell migration detected by scratch test; b The cell invasion detected by Transwell assay; c Representative images of xenografted tumors in nude mice of each group; d Xenografted tumor volume in nude mice of each group; e Xenografted tumor weight in nude mice of each group; The measurement data were expressed as mean ± standard deviation. The t test was used for two-group comparison. ^ vs the sh-NC group, P < 0.05

Journal: Cancer Cell International

Article Title: Silenced lncRNA DDX11-AS1 or up-regulated microRNA-34a-3p inhibits malignant phenotypes of hepatocellular carcinoma cells via suppression of TRAF5

doi: 10.1186/s12935-021-01847-6

Figure Lengend Snippet: Reduced DDX11-AS1 inhibits the migration, invasion of HCC cells, promotes and represses the tumor growth in nude mice. a The cell migration detected by scratch test; b The cell invasion detected by Transwell assay; c Representative images of xenografted tumors in nude mice of each group; d Xenografted tumor volume in nude mice of each group; e Xenografted tumor weight in nude mice of each group; The measurement data were expressed as mean ± standard deviation. The t test was used for two-group comparison. ^ vs the sh-NC group, P < 0.05

Article Snippet: The lentivirus containing sh-DDX11-AS1 and sh-NC, miR-34a-3p mimic and its NC were purchased from GenePharma Co. Ltd. (Shanghai, China). pcDDX11-AS1 was constructed by RiboBio Co., Ltd. (Guangdong, China).

Techniques: Migration, Transwell Assay, Standard Deviation

Reduced DDX11-AS1 increases miR-34a-3p to reduce TRAF5 expression. a DDX11-AS1, miR-34a-3p and TRAF5 expression detected by RT-qPCR; b TRAF5 protein bands; c TRAF5 protein expression detected by western blot analysis; d The binding sites of DDX11-AS1 and miR-34a-3p predicted via bioinformatics website https://cm.jefferson.edu/rna22/Precomputed/ ; e The regulatory relationship between DDX11-AS1 and miR-34a-3p verified via dual luciferase reporter gene assay; f The binding relationship between DDX11-AS1 and miR-34a-3p verified via RNA-pull down assay; g The targeting relationship between miR-34a-3p and TRAF5 predicted via bioinformatics website https://cm.jefferson.edu/rna22/Precomputed/ ; h The targeting relationship between miR-34a-3p and TRAF5 verified via luciferase activity assay; The measurement data were expressed as mean ± standard deviation. One-way ANOVA was used for comparison among multiple groups, and Tukey’s post hoc test was used for pairwise comparison after ANOVA analysis. ^ vs the sh-NC group, P < 0.05; # vs the mimic NC group, P < 0.05

Journal: Cancer Cell International

Article Title: Silenced lncRNA DDX11-AS1 or up-regulated microRNA-34a-3p inhibits malignant phenotypes of hepatocellular carcinoma cells via suppression of TRAF5

doi: 10.1186/s12935-021-01847-6

Figure Lengend Snippet: Reduced DDX11-AS1 increases miR-34a-3p to reduce TRAF5 expression. a DDX11-AS1, miR-34a-3p and TRAF5 expression detected by RT-qPCR; b TRAF5 protein bands; c TRAF5 protein expression detected by western blot analysis; d The binding sites of DDX11-AS1 and miR-34a-3p predicted via bioinformatics website https://cm.jefferson.edu/rna22/Precomputed/ ; e The regulatory relationship between DDX11-AS1 and miR-34a-3p verified via dual luciferase reporter gene assay; f The binding relationship between DDX11-AS1 and miR-34a-3p verified via RNA-pull down assay; g The targeting relationship between miR-34a-3p and TRAF5 predicted via bioinformatics website https://cm.jefferson.edu/rna22/Precomputed/ ; h The targeting relationship between miR-34a-3p and TRAF5 verified via luciferase activity assay; The measurement data were expressed as mean ± standard deviation. One-way ANOVA was used for comparison among multiple groups, and Tukey’s post hoc test was used for pairwise comparison after ANOVA analysis. ^ vs the sh-NC group, P < 0.05; # vs the mimic NC group, P < 0.05

Article Snippet: The lentivirus containing sh-DDX11-AS1 and sh-NC, miR-34a-3p mimic and its NC were purchased from GenePharma Co. Ltd. (Shanghai, China). pcDDX11-AS1 was constructed by RiboBio Co., Ltd. (Guangdong, China).

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Binding Assay, Luciferase, Reporter Gene Assay, Pull Down Assay, Activity Assay, Standard Deviation

DDX11-AS1 modulates the growth and metastasis of HCC cells through miR-34a-3p/TRAF5 axis. a , b DDX11-AS1, miR-34a-3p and TRAF5 expression after co-transfection detected by RT-qPCR; c TRAF5 protein expression after co-transfection detected by western blot analysis; d The cell growth curve detected by CCK-8 assay; e The cell proliferation rate detected via colony formation assay; f The apoptosis rate detected via Annexin V-FITC/PI double staining; The measurement data were expressed as mean ± standard deviation. t test was used for comparison between two groups, One-way ANOVA for comparison among multiple groups, and Tukey’s post hoc test for pairwise comparison. & vs the pcDDX11-AS1 + mimic NC group, P < 0.05

Journal: Cancer Cell International

Article Title: Silenced lncRNA DDX11-AS1 or up-regulated microRNA-34a-3p inhibits malignant phenotypes of hepatocellular carcinoma cells via suppression of TRAF5

doi: 10.1186/s12935-021-01847-6

Figure Lengend Snippet: DDX11-AS1 modulates the growth and metastasis of HCC cells through miR-34a-3p/TRAF5 axis. a , b DDX11-AS1, miR-34a-3p and TRAF5 expression after co-transfection detected by RT-qPCR; c TRAF5 protein expression after co-transfection detected by western blot analysis; d The cell growth curve detected by CCK-8 assay; e The cell proliferation rate detected via colony formation assay; f The apoptosis rate detected via Annexin V-FITC/PI double staining; The measurement data were expressed as mean ± standard deviation. t test was used for comparison between two groups, One-way ANOVA for comparison among multiple groups, and Tukey’s post hoc test for pairwise comparison. & vs the pcDDX11-AS1 + mimic NC group, P < 0.05

Article Snippet: The lentivirus containing sh-DDX11-AS1 and sh-NC, miR-34a-3p mimic and its NC were purchased from GenePharma Co. Ltd. (Shanghai, China). pcDDX11-AS1 was constructed by RiboBio Co., Ltd. (Guangdong, China).

Techniques: Expressing, Cotransfection, Quantitative RT-PCR, Western Blot, CCK-8 Assay, Colony Assay, Double Staining, Standard Deviation

DDX11-AS1 modulates the growth and metastasis of HCC cells through miR-34a-3p/TRAF5 axis. a , b DDX11-AS1, miR-34a-3p and TRAF5 expression after co-transfection detected by RT-qPCR; c TRAF5 protein expression after co-transfection detected by western blot analysis; d The cell growth curve detected by CCK-8 assay; e The cell proliferation rate detected via colony formation assay; f The apoptosis rate detected via Annexin V-FITC/PI double staining; The measurement data were expressed as mean ± standard deviation. The measurement data were expressed as mean ± standard deviation. t test was used for comparison between two groups, One-way ANOVA for comparison among multiple groups, and Tukey’s post hoc test for pairwise comparison. + vs the pcDDX11-AS1 + sh-NC group, P < 0.05

Journal: Cancer Cell International

Article Title: Silenced lncRNA DDX11-AS1 or up-regulated microRNA-34a-3p inhibits malignant phenotypes of hepatocellular carcinoma cells via suppression of TRAF5

doi: 10.1186/s12935-021-01847-6

Figure Lengend Snippet: DDX11-AS1 modulates the growth and metastasis of HCC cells through miR-34a-3p/TRAF5 axis. a , b DDX11-AS1, miR-34a-3p and TRAF5 expression after co-transfection detected by RT-qPCR; c TRAF5 protein expression after co-transfection detected by western blot analysis; d The cell growth curve detected by CCK-8 assay; e The cell proliferation rate detected via colony formation assay; f The apoptosis rate detected via Annexin V-FITC/PI double staining; The measurement data were expressed as mean ± standard deviation. The measurement data were expressed as mean ± standard deviation. t test was used for comparison between two groups, One-way ANOVA for comparison among multiple groups, and Tukey’s post hoc test for pairwise comparison. + vs the pcDDX11-AS1 + sh-NC group, P < 0.05

Article Snippet: The lentivirus containing sh-DDX11-AS1 and sh-NC, miR-34a-3p mimic and its NC were purchased from GenePharma Co. Ltd. (Shanghai, China). pcDDX11-AS1 was constructed by RiboBio Co., Ltd. (Guangdong, China).

Techniques: Expressing, Cotransfection, Quantitative RT-PCR, Western Blot, CCK-8 Assay, Colony Assay, Double Staining, Standard Deviation

DDX11-AS1 modulates the growth and metastasis of HCC cells through miR-34a-3p/TRAF5 axis. a The cell migration detected by scratch test; b The cell invasion detected by Transwell assay; c Representative images of xenografted tumors in nude mice; d Xenografted tumor volume in nude mice of each group; e Xenografted tumor weight in nude mice of each group; The measurement data were expressed as mean ± standard deviation. The measurement data were expressed as mean ± standard deviation. t test was used for comparison between two groups, One-way ANOVA for comparison among multiple groups, and Tukey’s post hoc test for pairwise comparison. & vs the pcDDX11-AS1 + mimic NC group, P < 0.05

Journal: Cancer Cell International

Article Title: Silenced lncRNA DDX11-AS1 or up-regulated microRNA-34a-3p inhibits malignant phenotypes of hepatocellular carcinoma cells via suppression of TRAF5

doi: 10.1186/s12935-021-01847-6

Figure Lengend Snippet: DDX11-AS1 modulates the growth and metastasis of HCC cells through miR-34a-3p/TRAF5 axis. a The cell migration detected by scratch test; b The cell invasion detected by Transwell assay; c Representative images of xenografted tumors in nude mice; d Xenografted tumor volume in nude mice of each group; e Xenografted tumor weight in nude mice of each group; The measurement data were expressed as mean ± standard deviation. The measurement data were expressed as mean ± standard deviation. t test was used for comparison between two groups, One-way ANOVA for comparison among multiple groups, and Tukey’s post hoc test for pairwise comparison. & vs the pcDDX11-AS1 + mimic NC group, P < 0.05

Article Snippet: The lentivirus containing sh-DDX11-AS1 and sh-NC, miR-34a-3p mimic and its NC were purchased from GenePharma Co. Ltd. (Shanghai, China). pcDDX11-AS1 was constructed by RiboBio Co., Ltd. (Guangdong, China).

Techniques: Migration, Transwell Assay, Standard Deviation

DDX11-AS1 modulates the growth and metastasis of HCC cells through miR-34a-3p/TRAF5 axis. a The cell migration detected by scratch test; b The cell invasion detected by Transwell assay; c Representative images of xenografted tumors in nude mice of each group; d Xenografted tumor volume in nude mice of each group; e Xenografted tumor weight in nude mice of each group; The measurement data were expressed as mean ± standard deviation. The measurement data were expressed as mean ± standard deviation. t test was used for comparison between two groups, One-way ANOVA for comparison among multiple groups, and Tukey’s post hoc test for pairwise comparison. + vs the pcDDX11-AS1 + sh-NC group, P < 0.05

Journal: Cancer Cell International

Article Title: Silenced lncRNA DDX11-AS1 or up-regulated microRNA-34a-3p inhibits malignant phenotypes of hepatocellular carcinoma cells via suppression of TRAF5

doi: 10.1186/s12935-021-01847-6

Figure Lengend Snippet: DDX11-AS1 modulates the growth and metastasis of HCC cells through miR-34a-3p/TRAF5 axis. a The cell migration detected by scratch test; b The cell invasion detected by Transwell assay; c Representative images of xenografted tumors in nude mice of each group; d Xenografted tumor volume in nude mice of each group; e Xenografted tumor weight in nude mice of each group; The measurement data were expressed as mean ± standard deviation. The measurement data were expressed as mean ± standard deviation. t test was used for comparison between two groups, One-way ANOVA for comparison among multiple groups, and Tukey’s post hoc test for pairwise comparison. + vs the pcDDX11-AS1 + sh-NC group, P < 0.05

Article Snippet: The lentivirus containing sh-DDX11-AS1 and sh-NC, miR-34a-3p mimic and its NC were purchased from GenePharma Co. Ltd. (Shanghai, China). pcDDX11-AS1 was constructed by RiboBio Co., Ltd. (Guangdong, China).

Techniques: Migration, Transwell Assay, Standard Deviation